1TV3

Crystal structure of the N-methyl-hydroxylamine MtmB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-20
Detector	MARRESEARCH
Wavelength(s)	1.08000
Spacegroup name	P 63 2 2
Unit cell lengths	157.987, 157.987, 136.914
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 2.200
R-factor	0.146
Rwork	0.146
R-free	0.16800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nth
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.106	0.335
Number of reflections	51381
<I/σ(I)>	17.3	4.9
Completeness [%]	99.9	100
Redundancy	6.1	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	NACL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	NACL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	NACL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K