1TRJ

Homology Model of Yeast RACK1 Protein fitted into 11.7A cryo-EM map of Yeast 80S Ribosome

Summary for 1TRJ

• Entry DOI: 10.2210/pdb1trj/pdb
• Related: 1GOT 1S1H
• EMDB information: 1067
• Descriptor: Helix 39 of 18S rRNA, Helix 40 of 18S rRNA, Guanine nucleotide-binding protein beta subunit-like protein (3 entities in total)
• Functional Keywords: rack1, ribosome, homology model, signaling protein
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Total number of polymer chains: 3
• Total formula weight: 56348.85

Authors

Sengupta, J.,Nilsson, J.,Gursky, R.,Spahn, C.M.,Nissen, P.,Frank, J. (deposition date: 2004-06-21, release date: 2004-09-28, Last modification date: 2024-02-14)

Primary citation

Sengupta, J.,Nilsson, J.,Gursky, R.,Spahn, C.M.,Nissen, P.,Frank, J.
Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM
Nat.Struct.Mol.Biol., 11:957-962, 2004

PubMed Abstract: RACK1 serves as a scaffold protein for a wide range of kinases and membrane-bound receptors. It is a WD-repeat family protein and is predicted to have a beta-propeller architecture with seven blades like a Gbeta protein. Mass spectrometry studies have identified its association with the small subunit of eukaryotic ribosomes and, most recently, it has been shown to regulate initiation by recruiting protein kinase C to the 40S subunit. Here we present the results of a cryo-EM study of the 80S ribosome that positively locate RACK1 on the head region of the 40S subunit, in the immediate vicinity of the mRNA exit channel. One face of RACK1 exposes the WD-repeats as a platform for interactions with kinases and receptors. Using this platform, RACK1 can recruit other proteins to the ribosome.

PubMed: 15334071
DOI: 10.1038/nsmb822

Experimental method

ELECTRON MICROSCOPY (11.7 Å)