1TGH

TATA BINDING PROTEIN (TBP)/DNA COMPLEX

Summary for 1TGH

• Entry DOI: 10.2210/pdb1tgh/pdb
• Descriptor: DNA (5'-D(*CP*GP*TP*AP*TP*AP*TP*AP*TP*AP*CP*G)-3'), PROTEIN (TATA BINDING PROTEIN (TBP)) (3 entities in total)
• Functional Keywords: transcription factor, dna binding protein, dna, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P20226
• Total number of polymer chains: 3
• Total formula weight: 28124.46

Authors

Juo, Z.S.,Dickerson, R.E. (deposition date: 1996-02-13, release date: 1996-08-01, Last modification date: 2024-02-14)

Primary citation

Juo, Z.S.,Chiu, T.K.,Leiberman, P.M.,Baikalov, I.,Berk, A.J.,Dickerson, R.E.
How proteins recognize the TATA box.
J.Mol.Biol., 261:239-254, 1996

PubMed Abstract: The crystal structure of a complex of human TATA-binding protein with TATA-sequence DNA has been solved, complementing earlier TBP/DNA analyses from Saccharomyces cerevisiae and Arabidopsis thaliana. Special insight into TATA box specificity is provided by considering the TBP/DNA complex, not as a protein molecule with bound DNA, but as a DNA duplex with a particularly large minor groove ligand. This point of view provides explanations for: (1) why T.A base-pairs are required rather than C.G; (2) why an alternation of T and A bases is needed; (3) how TBP recognizes the upstream and downstream ends of the TATA box in order to bind properly; and (4) why the second half of the TATA box can be more variable than the first.

PubMed: 8757291
DOI: 10.1006/jmbi.1996.0456

Experimental method

X-RAY DIFFRACTION (2.9 Å)