1TGH
TATA BINDING PROTEIN (TBP)/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 92 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-19 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.965, 67.397, 86.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.2145 * |
| Rwork | 0.214 |
| R-free | 0.29460 * |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.055 |
| Number of reflections | 9013 * |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 4 * | pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hTBPc and DNA | 0.6 (mM) | |
| 10 | 1 | reservoir | DTT | 5 (mM) | |
| 11 | 1 | reservoir | 1 (mM) | ||
| 12 | 1 | reservoir | PEG8000 | 5 (%) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | ammonium acetate | 350 (mM) | |
| 4 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 5 | 1 | drop | DTT | 5 (mM) | |
| 6 | 1 | drop | 1 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 8 | 1 | reservoir | ammonium acetate | 350 (mM) | |
| 9 | 1 | reservoir | glycerol | 10 (%(v/v)) |
