1T8E

T7 DNA Polymerase Ternary Complex with dCTP at the Insertion Site.

Summary for 1T8E

Descriptor5'-D(P*CP*GP*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(2DT))-3', 25-MER, DNA polymerase, ... (10 entities in total)
Functional Keywordsprotein, dna, transferase, transferase-electron transport-dna complex, transferase/electron transport/dna
Biological sourceEnterobacteria phage T7
More
Total number of polymer chains4
Total molecular weight106552.31
Authors
Brieba, L.G.,Eichman, B.F.,Kokoska, R.J.,Doublie, S.,Kunkel, T.A.,Ellenberger, T. (deposition date: 2004-05-12, release date: 2004-10-12, Last modification date: 2011-07-13)
Primary citation
Brieba, L.G.,Eichman, B.F.,Kokoska, R.J.,Doublie, S.,Kunkel, T.A.,Ellenberger, T.
Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.
Embo J., 23:3452-3461, 2004
PubMed: 15297882 (PDB entries with the same primary citation)
DOI: 10.1038/sj.emboj.7600354
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.54 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.251151.4%4.1%4.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report