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1T8E

T7 DNA Polymerase Ternary Complex with dCTP at the Insertion Site.

Summary for 1T8E
Entry DOI10.2210/pdb1t8e/pdb
Descriptor5'-D(P*CP*GP*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(2DT))-3', 25-MER, DNA polymerase, ... (10 entities in total)
Functional Keywordsprotein, dna, transferase, transferase-electron transport-dna complex, transferase/electron transport/dna
Biological sourceEnterobacteria phage T7
More
Total number of polymer chains4
Total formula weight106552.31
Authors
Brieba, L.G.,Eichman, B.F.,Kokoska, R.J.,Doublie, S.,Kunkel, T.A.,Ellenberger, T. (deposition date: 2004-05-12, release date: 2004-10-12, Last modification date: 2024-02-14)
Primary citationBrieba, L.G.,Eichman, B.F.,Kokoska, R.J.,Doublie, S.,Kunkel, T.A.,Ellenberger, T.
Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.
Embo J., 23:3452-3461, 2004
Cited by
PubMed Abstract: Accurate DNA replication involves polymerases with high nucleotide selectivity and proofreading activity. We show here why both fidelity mechanisms fail when normally accurate T7 DNA polymerase bypasses the common oxidative lesion 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8oG). The crystal structure of the polymerase with 8oG templating dC insertion shows that the O8 oxygen is tolerated by strong kinking of the DNA template. A model of a corresponding structure with dATP predicts steric and electrostatic clashes that would reduce but not eliminate insertion of dA. The structure of a postinsertional complex shows 8oG(syn).dA (anti) in a Hoogsteen-like base pair at the 3' terminus, and polymerase interactions with the minor groove surface of the mismatch that mimic those with undamaged, matched base pairs. This explains why translesion synthesis is permitted without proofreading of an 8oG.dA mismatch, thus providing insight into the high mutagenic potential of 8oG.
PubMed: 15297882
DOI: 10.1038/sj.emboj.7600354
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.54 Å)
Structure validation

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