1T76

Crystal structure of the androgen receptor ligand binding domain in complex with a WxxVW motif

Summary for 1T76

• Entry DOI: 10.2210/pdb1t76/pdb
• Related: 1T73 1T74 1T79 1T7F 1T7M 1T7R 1T7T
• Descriptor: Androgen receptor, WxxVW motif peptide, SULFATE ION, ... (7 entities in total)
• Functional Keywords: nuclear receptor, transcription factor, ligand binding domain, af-2, androgen, testosterone, dht, alpha-helical sandwich, hormone-growth factor receptor complex, hormone/growth factor receptor
• Biological source: Pan troglodytes (chimpanzee); More
• Cellular location: Nucleus (By similarity): O97775
• Total number of polymer chains: 2
• Total formula weight: 33187.49

Authors

Hur, E.,Pfaff, S.J.,Payne, E.S.,Gron, H.,Buehrer, B.M.,Fletterick, R.J. (deposition date: 2004-05-08, release date: 2004-08-31, Last modification date: 2024-02-14)

Primary citation

Hur, E.,Pfaff, S.J.,Payne, E.S.,Gron, H.,Buehrer, B.M.,Fletterick, R.J.
Recognition and accommodation at the androgen receptor coactivator binding interface.
Plos Biol., 2:E274-E274, 2004

PubMed Abstract: Prostate cancer is a leading killer of men in the industrialized world. Underlying this disease is the aberrant action of the androgen receptor (AR). AR is distinguished from other nuclear receptors in that after hormone binding, it preferentially responds to a specialized set of coactivators bearing aromatic-rich motifs, while responding poorly to coactivators bearing the leucine-rich "NR box" motifs favored by other nuclear receptors. Under normal conditions, interactions with these AR-specific coactivators through aromatic-rich motifs underlie targeted gene transcription. However, during prostate cancer, abnormal association with such coactivators, as well as with coactivators containing canonical leucine-rich motifs, promotes disease progression. To understand the paradox of this unusual selectivity, we have derived a complete set of peptide motifs that interact with AR using phage display. Binding affinities were measured for a selected set of these peptides and their interactions with AR determined by X-ray crystallography. Structures of AR in complex with FxxLF, LxxLL, FxxLW, WxxLF, WxxVW, FxxFF, and FxxYF motifs reveal a changing surface of the AR coactivator binding interface that permits accommodation of both AR-specific aromatic-rich motifs and canonical leucine-rich motifs. Induced fit provides perfect mating of the motifs representing the known family of AR coactivators and suggests a framework for the design of AR coactivator antagonists.

PubMed: 15328534
DOI: 10.1371/journal.pbio.0020274

Experimental method

X-RAY DIFFRACTION (2.1 Å)