1SXJ

Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)

1SXJ の概要

• エントリーDOI: 10.2210/pdb1sxj/pdb
• 関連するPDBエントリー: 1PLQ
• 分子名称: Activator 1 95 kDa subunit, Activator 1 37 kDa subunit, Activator 1 40 kDa subunit, ... (9 entities in total)
• 機能のキーワード: clamp loader, processivity clamp, dna sliding clamp, aaa+ atpase, dna polymerase, dna-binding protein, replication
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus (Probable): P38630 P40339 P38629 P40348 P38251; Nucleus: P15873
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 309282.41

構造登録者

Bowman, G.D.,O'Donnell, M.,Kuriyan, J. (登録日: 2004-03-30, 公開日: 2004-06-22, 最終更新日: 2024-10-09)

主引用文献

Bowman, G.D.,O'Donnell, M.,Kuriyan, J.
Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex.
Nature, 429:724-730, 2004

PubMed Abstract: Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA). Tight interfacial coordination of the ATP analogue ATP-gammaS by RFC results in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for primed DNA within the central hole of PCNA reveals a striking correspondence between the RFC spiral and the grooves of the DNA double helix. This model, in which the clamp loader complex locks onto primed DNA in a screw-cap-like arrangement, provides a simple explanation for the process by which the engagement of primer-template junctions by the RFC:PCNA complex results in ATP hydrolysis and release of the sliding clamp on DNA.

PubMed: 15201901
DOI: 10.1038/nature02585

実験手法

X-RAY DIFFRACTION (2.85 Å)