1SMY

Structural basis for transcription regulation by alarmone ppGpp

1SMY の概要

• エントリーDOI: 10.2210/pdb1smy/pdb
• 関連するPDBエントリー: 1IW7
• 分子名称: DNA-directed RNA polymerase alpha chain, DNA-directed RNA polymerase beta chain, DNA-directed RNA polymerase beta' chain, ... (9 entities in total)
• 機能のキーワード: rna polymerase holoenzyme, guanosine-tetraphosphate, ppgpp, transcription regulation, riken structural genomics/proteomics initiative, rsgi, structural genomics, transferase
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 863537.34

構造登録者

Artsimovitch, I.,Patlan, V.,Sekine, S.,Vassylyeva, M.N.,Hosaka, T.,Ochi, K.,Yokoyama, S.,Vassylyev, D.G.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2004-03-10, 公開日: 2004-05-18, 最終更新日: 2023-10-25)

主引用文献

Artsimovitch, I.,Patlan, V.,Sekine, S.,Vassylyeva, M.N.,Hosaka, T.,Ochi, K.,Yokoyama, S.,Vassylyev, D.G.
Structural basis for transcription regulation by alarmone ppGpp
Cell(Cambridge,Mass.), 117:299-310, 2004

PubMed Abstract: Guanosine-tetraphosphate (ppGpp) is a major regulator of stringent control, an adaptive response of bacteria to amino acid starvation. The 2.7 A resolution structure of the Thermus thermophilus RNA polymerase (RNAP) holoenzyme in complex with ppGpp reveals that ppGpp binds to the same site near the active center in both independent RNAP molecules in the crystal but in strikingly distinct orientations. Binding is symmetrical with respect to the two diphosphates of ppGpp and is relaxed with respect to the orientation of the nucleotide base. Different modes of ppGpp binding are coupled with asymmetry of the active site configurations. The results suggest that base pairing of ppGpp with cytosines in the nontemplate DNA strand might be an essential component of transcription control by ppGpp. We present experimental evidence highlighting the importance of base-specific contacts between ppGpp and specific cytosine residues during both transcription initiation and elongation.

PubMed: 15109491
DOI: 10.1016/S0092-8674(04)00401-5

実験手法

X-RAY DIFFRACTION (2.7 Å)