1SM3

CRYSTAL STRUCTURE OF THE TUMOR SPECIFIC ANTIBODY SM3 COMPLEX WITH ITS PEPTIDE EPITOPE

1SM3 の概要

• エントリーDOI: 10.2210/pdb1sm3/pdb
• 分子名称: SM3 ANTIBODY, PEPTIDE EPITOPE, CADMIUM ION, ... (6 entities in total)
• 機能のキーワード: antibody, peptide antigen, antitumor antibody, complex (antibody-peptide epitope), complex (antibody-peptide epitope) complex, complex (antibody/peptide epitope)
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Apical cell membrane; Single-pass type I membrane protein. Isoform 5: Secreted. Isoform 7: Secreted. Isoform 9: Secreted. Mucin-1 subunit beta: Cell membrane: P15941
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 48838.30

構造登録者

Dokurno, P.,Bates, P.A.,Band, H.A.,Stewart, L.M.D.,Lally, J.M.,Burchell, J.M.,Taylor-Papadimitriou, J.,Sternberg, M.J.E.,Snary, D.,Freemont, P.S. (登録日: 1997-12-23, 公開日: 1999-03-23, 最終更新日: 2024-11-20)

主引用文献

Dokurno, P.,Bates, P.A.,Band, H.A.,Stewart, L.M.,Lally, J.M.,Burchell, J.M.,Taylor-Papadimitriou, J.,Snary, D.,Sternberg, M.J.,Freemont, P.S.
Crystal structure at 1.95 A resolution of the breast tumour-specific antibody SM3 complexed with its peptide epitope reveals novel hypervariable loop recognition.
J.Mol.Biol., 284:713-728, 1998

PubMed Abstract: The anti-breast tumour antibody SM3 has a high selectivity in reacting specifically with carcinoma-associated mucin. SM3 recognises the core repeating motif (Pro-Asp-Thr-Arg-Pro) of aberrantly glycosylated epithelial mucin MUC1, and has potential as a therapeutic and diagnostic tool. Here we report the crystal structure of the Fab fragment of SM3 in complex with a 13-residue MUC1 peptide antigen (Thr1P-Ser2P-Ala3P-Pro4P-Asp5P-Thr6P -Arg7P-Pro8P-Ala9P-Pro10P-Gly11P- Ser12P-Thr13P). The SM3-MUC1 peptide structure was solved by molecular replacement, and the current model is refined at 1.95 A resolution with an R-factor of 21.3% and R-free 28.3%. The MUC1 peptide is bound both by non-polar interactions and hydrogen bonds in an elongated groove in the antibody-combining site through interactions with Complimentarity Determining Regions (CDRs), three of the light chain (L1, L2, L3) and two of the heavy chain (H1 and H3). The conformation of the peptide is mainly extended with no discernable standard secondary structure. There is a single non-proline cis-peptide bond in H3 (Val95H-Gly96H-Gln97H-Phe98H-Ala101H-Ty r102H) between Gly96H and Gln97H, which appears to play a role in SM3-peptide antigen interactions, and represents the first such example within an antibody hypervariable loop. The SM3-MUC1 peptide structure has implications for rational therapeutic and diagnostic antibody engineering.

PubMed: 9826510
DOI: 10.1006/jmbi.1998.2209

実験手法

X-RAY DIFFRACTION (1.95 Å)