1S0O

Snapshots of replication through an abasic lesion: structural basis for base substitution and frameshift

1S0O の概要

• エントリーDOI: 10.2210/pdb1s0o/pdb
• 関連するPDBエントリー: 1JX4 1RYR 1RYS 1S0M 1S0N 1S10
• 分子名称: 5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP*CP*TP*C)-3', 5'-D(*TP*CP*AP*GP*TP*AP*GP*TP*CP*CP*TP*TP*CP*CP*CP*CP*C)-3', DNA polymerase IV, ... (7 entities in total)
• 機能のキーワード: abaic lesion, lesion bypass, polymerase, transferase-dna complex, transferase/dna
• 由来する生物種: Sulfolobus solfataricus
• 細胞内の位置: Cytoplasm : Q97W02
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 100081.06

構造登録者

Ling, H.,Boudsocq, F.,Woodgate, R.,Yang, W. (登録日: 2003-12-31, 公開日: 2004-03-30, 最終更新日: 2023-08-23)

主引用文献

Ling, H.,Boudsocq, F.,Woodgate, R.,Yang, W.
Snapshots of Replication through an Abasic Lesion; Structural Basis for Base Substitutions and Frameshifts.
Mol.Cell, 13:751-762, 2004

PubMed Abstract: Dpo4 from S. Solfataricus, a DinB-like Y family polymerase, efficiently replicates DNA past an abasic lesion. We have determined crystal structures of Dpo4 complexed with five different abasic site-containing DNA substrates and find that translesion synthesis is template directed with the abasic site looped out and the incoming nucleotide is opposite the base 5' to the lesion. The ensuing DNA synthesis generates a -1 frameshift when the abasic site remains extrahelical. Template realignment during primer extension is also observed, resulting in base substitutions or even +1 frameshifts. In the case of a +1 frameshift, the extra nucleotide is accommodated in the solvent-exposed minor groove. In addition, the structure of an unproductive Dpo4 ternary complex suggests that the flexible little finger domain facilitates DNA orientation and translocation during translesion synthesis.

PubMed: 15023344
DOI: 10.1016/S1097-2765(04)00101-7

実験手法

X-RAY DIFFRACTION (2.1 Å)