1RLD

SOLID-STATE PHASE TRANSITION IN THE CRYSTAL STRUCTURE OF RIBULOSE 1,5-BIPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE

Summary for 1RLD

• Entry DOI: 10.2210/pdb1rld/pdb
• Descriptor: RIBULOSE 1,5 BISPHOSPHATE CARBOXYLASE/OXYGENASE (LARGE CHAIN), RIBULOSE 1,5 BISPHOSPHATE CARBOXYLASE/OXYGENASE (SMALL CHAIN) (2 entities in total)
• Functional Keywords: lyase(carbon-carbon)
• Biological source: Nicotiana tabacum (common tobacco); More
• Cellular location: Plastid, chloroplast: P00876 P69249
• Total number of polymer chains: 4
• Total formula weight: 128389.71

Authors

Zhang, K.Y.J.,Eisenberg, D. (deposition date: 1993-12-10, release date: 1994-04-30, Last modification date: 2024-10-30)

Primary citation

Zhang, K.Y.,Eisenberg, D.
Solid-state phase transition in the crystal structure of ribulose 1,5-bisphosphate carboxylase/oxygenase.
Acta Crystallogr.,Sect.D, 50:258-262, 1994

PubMed Abstract: The crystal structure is described of ribulose 1,5-bisphosphate carboxylase/oxygenase in a new crystal form. This new form (form V) was obtained from a previously known crystal form (form III) through a solid-state phase transition. The solid-state phase transition was brought about by transferring the crystal from a high-salt low-pH mother liquor to a low-salt high-pH synthetic mother liquor. The interplay of electrostatic repulsion and osmotic pressure induced a unit-cell shrinkage of 24 A along the c axis and expansion of 4 A along the a and b axes. The space group also changed from I422 to I4. The new crystal form was shown to be more resistant to X-ray radiation damage, which suggests the effect of crystal stabilization by non-penetrating molecules. The structure of ribulose 1,5-bisphosphate carboxylase/oxygenase in the new crystal form is compared with that of the old crystal form.

PubMed: 15299436
DOI: 10.1107/S090744499301306X

Experimental method

X-RAY DIFFRACTION (2.5 Å)