1RBJ

RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))

Summary for 1RBJ

• Entry DOI: 10.2210/pdb1rbj/pdb
• Descriptor: DNA (5'-D(*AP*AP*AP*A)-3'), PROTEIN (RIBONUCLEASE B (E.C.3.1.27.5)) (2 entities in total)
• Functional Keywords: protein-dna complex, hydrolase-dna complex, hydrolase/dna
• Biological source: Bos taurus (cattle)
• Cellular location: Secreted: P61823
• Total number of polymer chains: 2
• Total formula weight: 14916.20

Authors

Ko, T.-P.,Williams, R.,McPherson, A. (deposition date: 1995-05-22, release date: 1995-12-07, Last modification date: 2024-10-16)

Primary citation

Ko, T.P.,Williams, R.,McPherson, A.
Structure of a ribonuclease B+d(pA)4 complex.
Acta Crystallogr.,Sect.D, 52:160-164, 1996

PubMed Abstract: The structure of a tetragonal crystal of bovine pancreatic RNase B complexed with d(pA)(4) was determined by molecular replacement and difference Fourier methods. This crystal belongs to space group P4(1)2(1)2 and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include any polysaccharide residues or solvent molecules. After refinement at 2.7 A, the R value was 0.163 with acceptable stereochemistry. The model illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.

PubMed: 15299737
DOI: 10.1107/S0907444995009127

Experimental method

X-RAY DIFFRACTION (2.7 Å)