1R9U
Refined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplings
Summary for 1R9U
| Entry DOI | 10.2210/pdb1r9u/pdb |
| Related | 1AMT 1DLZ 1EE7 1GQ0 1IH9 1JOH 1M24 1OB4 1OB6 1OB7 |
| Related PRD ID | PRD_000159 |
| Descriptor | ZERVAMICIN IIB (1 entity in total) |
| Functional Keywords | zrevamicin, bifurcated hydrogen bond, peptaibol, antibacterial, antifungal, antibiotic, bent helix |
| Biological source | EMERICELLOPSIS SALMOSYNNEMATA |
| Total number of polymer chains | 1 |
| Total formula weight | 1823.18 |
| Authors | Shenkarev, Z.O.,Balashova, T.A.,Yakimenko, Z.A.,Ovchinnikova, T.V.,Arseniev, A.S. (deposition date: 2003-10-31, release date: 2004-11-09, Last modification date: 2025-03-26) |
| Primary citation | Ovchinnikova, T.V.,Shenkarev, Z.O.,Yakimenko, Z.A.,Svishcheva, N.V.,Tagaev, A.A.,Skladnev, D.A.,Arseniev, A.S. Biosynthetic Uniform 13C,15N-Labelling of Zervamicin Iib. Complete 13C and 15N NMR Assignment. J.Pept.Sci., 9:817-826, 2003 Cited by PubMed Abstract: Zervamicin IIB is a member of the alpha-aminoisobutyric acid containing peptaibol antibiotics. A new procedure for the biosynthetic preparation of the uniformly 13C- and 15N-enriched peptaibol is described This compound was isolated from the biomass of the fungus-producer Emericellopsis salmosynnemata strain 336 IMI 58330 obtained upon cultivation in the totally 13C, 15N-labelled complete medium. To prepare such a medium the autolysed biomass and the exopolysaccharides of the obligate methylotrophic bacterium Methylobacillus flagellatus KT were used. This microorganism was grown in totally 13C, 15N-labelled minimal medium containing 13C-methanol and 15N-ammonium chloride as the only carbon and nitrogen sources. Preliminary NMR spectroscopic analysis indicated a high extent of isotope incorporation (> 90%) and led to the complete 13C- and 15N-NMR assignment including the stereospecific assignment of Aib residues methyl groups. The observed pattern of the structurally important secondary chemical shifts of 1H(alpha), 13C=O and 13C(alpha) agrees well with the previously determined structure of zervamicin IIB in methanol solution. PubMed: 14658801DOI: 10.1002/PSC.499 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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