1R3G

1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues

Summary for 1R3G

• Entry DOI: 10.2210/pdb1r3g/pdb
• Descriptor: 5'-D(*GP*CP*GP*TP*AP*(GMU)P*AP*CP*GP*C)-3'), MAGNESIUM ION (3 entities in total)
• Functional Keywords: dna
• Total number of polymer chains: 2
• Total formula weight: 6320.56

Authors

Prakash, T.P.,Puschl, A.,Lesnik, E.,Tereshko, V.,Egli, M.,Manoharan, M. (deposition date: 2003-10-01, release date: 2003-10-21, Last modification date: 2024-02-14)

Primary citation

Prakash, T.P.,Lesnik, E.,Mohan, V.,Tereshko, V.,Egli, M.,Manoharan, M.
2'-O-[2-(Guanidinium)ethyl]-Modified Oligonucleotides: Stabilizing Effect on Duplex and Triplex Structures.
Org.Lett., 6:1971-1974, 2004

PubMed Abstract: [structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.

PubMed: 15176796
DOI: 10.1021/ol0493249

Experimental method

X-RAY DIFFRACTION (1.16 Å)