1R3G
1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.111, 43.151, 45.558 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.160 |
R-factor | 0.134 |
Rwork | 0.132 |
R-free | 0.17400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.982 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.500 |
High resolution limit [Å] | 1.160 |
Rmerge | 0.060 |
Number of reflections | 17426 |
Completeness [%] | 97.4 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | Manoharan, M., (1999) J. Org. Chem., 64, 6468. * |