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1QVA

YEAST INITIATION FACTOR 4A N-TERMINAL DOMAIN

Summary for 1QVA
Entry DOI10.2210/pdb1qva/pdb
DescriptorINITIATION FACTOR 4A (2 entities in total)
Functional Keywordsrna helicase, dead box, eif4a, gene regulation
Biological sourceSaccharomyces cerevisiae (baker's yeast)
Cellular locationCytoplasm: P10081
Total number of polymer chains1
Total formula weight24949.70
Authors
Johnson, E.R.,McKay, D.B. (deposition date: 1999-07-07, release date: 1999-12-02, Last modification date: 2024-02-14)
Primary citationJohnson, E.R.,McKay, D.B.
Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase
RNA, 5:1526-1534, 1999
Cited by
PubMed Abstract: The eukaryotic translation initiation factor 4A (elF4A) is a representative of the DEAD-box RNA helicase protein family. We have solved the crystallographic structure of the amino-terminal domain (residues 1-223) of yeast elF4A. The domain is built around a core scaffold, a parallel alpha-beta motif with five beta strands, that is found in other RNA and DNA helicases, as well as in the RecA protein. The amino acid sequence motifs that are conserved within the helicase family are localized to the beta strand-->alpha helix junctions within the core. The core of the amino terminal domain of elF4A is amplified with additional structural elements that differ from those of other helicases. The phosphate binding loop (the Walker A motif) is in an unusual closed conformation. The crystallographic structure reveals specific interactions between amino acid residues of the phosphate binding loop, the DEAD motif, and the SAT motif, whose alteration is known to impair coupling between the ATPase cycle and the RNA unwinding activity of elF4A.
PubMed: 10606264
DOI: 10.1017/S1355838299991410
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.5 Å)
Structure validation

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