1QVA

YEAST INITIATION FACTOR 4A N-TERMINAL DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	1999-03-31
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	38.800, 74.300, 81.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.500
R-factor	0.223
Rwork	0.223
R-free	0.27400
RMSD bond length	0.007
RMSD bond angle	24.000 *
Data reduction software	QUANTUM (IV CCD SOFTWARE)
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.062	0.153 *
Total number of observations	29694 *
Number of reflections	8026 *
<I/σ(I)>	21.1
Completeness [%]	93.8 *	72.7 *
Redundancy	3.7	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9 *	20 *	POLYETHYLENE GLYCOL, POTASSIUM ACETATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG5000	30 (%)
2	1	reservoir		100 (mM)
3	1	reservoir	CHES	50 (mM)