1QQT

METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI

Summary for 1QQT

• Entry DOI: 10.2210/pdb1qqt/pdb
• Descriptor: METHIONYL-TRNA SYNTHETASE, ZINC ION (3 entities in total)
• Functional Keywords: rossmann fold, helix bundle, trna ligase, ligase
• Biological source: Escherichia coli
• Cellular location: Cytoplasm: P00959
• Total number of polymer chains: 1
• Total formula weight: 62908.43

Authors

Mechulam, Y.,Schmitt, E.,Maveyraud, L.,Zelwer, C.,Nureki, O.,Yokoyama, S.,Konno, M.,Blanquet, S. (deposition date: 1999-06-08, release date: 2000-01-01, Last modification date: 2024-02-14)

Primary citation

Mechulam, Y.,Schmitt, E.,Maveyraud, L.,Zelwer, C.,Nureki, O.,Yokoyama, S.,Konno, M.,Blanquet, S.
Crystal structure of Escherichia coli methionyl-tRNA synthetase highlights species-specific features.
J.Mol.Biol., 294:1287-1297, 1999

PubMed Abstract: The 3D structure of monomeric C-truncated Escherichia coli methionyl-tRNA synthetase, a class 1 aminoacyl-tRNA synthetase, has been solved at 2.0 A resolution. Remarkably, the polypeptide connecting the two halves of the Rossmann fold exposes two identical knuckles related by a 2-fold axis but with zinc in the distal knuckle only. Examination of available MetRS orthologs reveals four classes according to the number and zinc content of the putative knuckles. Extreme cases are exemplified by the MetRS of eucaryotic or archaeal origin, where two knuckles and two metal ions are expected, and by the mitochondrial enzymes, which are predicted to have one knuckle without metal ion.

PubMed: 10600385
DOI: 10.1006/jmbi.1999.3339

Experimental method

X-RAY DIFFRACTION (2.03 Å)