1QQT

METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-07-05
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	78.150, 46.300, 87.700
Unit cell angles	90.00, 109.06, 90.00

Refinement procedure

Resolution	20.000 - 2.030
R-factor	0.174
Rwork	0.170
R-free	0.21400
Structure solution method	MIR and molecular replacement
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.053 *	0.129
Number of reflections	38431
<I/σ(I)>	10.7
Completeness [%]	98.4	80
Redundancy	3.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	277	1.08M AMMONIUM CITRATE, 30MM POTASSIUM PHOSPHATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 277K, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	3.6 (mg/ml)
2	1	drop	ammonium citrate	1.08 (M)
3	1	drop	potassium phosphate	30 (mM)
4	1	drop	2-mercaptoethanol	4 (mM)