1QC1

CRYSTAL STRUCTURE OF THE SELF-FITTED B-DNA DECAMER D(CCGCCGGCGG)

Summary for 1QC1

• Entry DOI: 10.2210/pdb1qc1/pdb
• Descriptor: 5'-D(*CP*CP*GP*CP*CP*GP*GP*CP*GP*G)-3', MAGNESIUM ION (3 entities in total)
• Functional Keywords: b-dna double-helix, dna-dna recognition, dna
• Total number of polymer chains: 2
• Total formula weight: 6191.18

Authors

Timsit, Y.,Moras, D. (deposition date: 1999-05-14, release date: 1999-05-28, Last modification date: 2024-02-14)

Primary citation

Timsit, Y.,Moras, D.
DNA self-fitting: the double helix directs the geometry of its supramolecular assembly
EMBO J., 13:2737-2746, 1994

PubMed Abstract: Groove-backbone interaction is a natural and biologically relevant mechanism for the specific assembly of B-DNA double helices. Crystal engineering and crystal packing analysis of oligonucleotides of different sizes and sequences reveal that the sequence-dependent self-fitting of B-DNA helices is a dominant constraint for their ordered assembly. It can override the other intermolecular interactions and impose the overall geometry of the packing. Analysis of experimental examples of architectural motifs formed by the geometric combination of self-fitted DNA segments leads to general rules for DNA assembly. Like a directing piece for a supramolecular 'construction set', the double helix imposes a limited number of geometric solutions. These basic architectural constraints could direct, in a codified manner, the formation of higher-order structures. DNA architectural motifs exhibit new structural and electrostatic properties which could have some implications for their molecular recognition by proteins acting on DNA.

PubMed: 8026458

Experimental method

X-RAY DIFFRACTION (2.5 Å)