1QC1
CRYSTAL STRUCTURE OF THE SELF-FITTED B-DNA DECAMER D(CCGCCGGCGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 258 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | H 3 |
Unit cell lengths | 53.700, 53.700, 45.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.500 |
R-factor | 0.147 |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.078 |
Total number of observations | 6367 * |
Number of reflections | 1817 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 4 * | 30 % MPD, 1MM DNA DUPLEX, 1 MM SPERMINE, 20 MM MAGNESIUM ACETATE, 50 MM CACODYLATE BUFFER, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | SPERMINE | ||
3 | 1 | 1 | MAGNESIUM ACETATE | ||
4 | 1 | 1 | CACODYLATE BUFFER | ||
5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | cacodylate | 50 (mM) | |
2 | 1 | drop | dodecamer duplex | 1 (mM) | |
3 | 1 | drop | spermine tetrahydrochloride | 1.2 (mM) | |
4 | 1 | drop | magnesium acetate | 18 (mM) | |
5 | 1 | reservoir | 45 (%) |