1Q5H

Human dUTP Pyrophosphatase complex with dUDP

1Q5H の概要

• エントリーDOI: 10.2210/pdb1q5h/pdb
• 分子名称: dUTP pyrophosphatase, MAGNESIUM ION, DEOXYURIDINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: dna repair, enzyme-dna interactions, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Mitochondrion. Isoform 2: Nucleus: P33316
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 49921.62

構造登録者

Mol, C.D.,Harris, J.M.,McIntosh, E.M.,Tainer, J.A. (登録日: 2003-08-07, 公開日: 2003-08-19, 最終更新日: 2023-08-16)

主引用文献

Mol, C.D.,Harris, J.M.,McIntosh, E.M.,Tainer, J.A.
Human dUTP pyrophosphatase: uracil recognition by a Beta hairpin and active sites formed by three separate subunits
Structure, 4:1077-1092, 1996

PubMed Abstract: The essential enzyme dUTP pyrophosphatase (dUTPase) is exquisitely specific for dUTP and is critical for the fidelity of DNA replication and repair. dUTPase hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing dUTP levels and providing the dUMP for dTTP biosynthesis. A high cellular dTTP: dUTP ratio is essential to avoid uracil incorporation into DNA, which would lead to strand breaks and cell death. We report the first detailed atomic-resolution structure of a eukaryotic dUTPase, human dUTPase, and complexes with the uracil-containing deoxyribonucleotides, dUMP, dUDP and dUTP.

PubMed: 8805593
DOI: 10.1016/S0969-2126(96)00114-1

実験手法

X-RAY DIFFRACTION (2 Å)