1Q3G

MurA (Asp305Ala) liganded with tetrahedral reaction intermediate

1Q3G の概要

• エントリーDOI: 10.2210/pdb1q3g/pdb
• 関連するPDBエントリー: 1a2n 1ejc 1ejd 1eyn 1naw 1q36 1uae
• 分子名称: UDP-N-acetylglucosamine 1-carboxyvinyltransferase, 3'-1-CARBOXY-1-PHOSPHONOOXY-ETHOXY-URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: inside-out alpha-beta barrel, transferase
• 由来する生物種: Enterobacter cloacae
• 細胞内の位置: Cytoplasm : P33038
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 729965.79

構造登録者

Eschenburg, S.,Kabsch, W.,Healy, M.L.,Schonbrunn, E. (登録日: 2003-07-29, 公開日: 2003-12-16, 最終更新日: 2024-11-06)

主引用文献

Eschenburg, S.,Kabsch, W.,Healy, M.L.,Schonbrunn, E.
A New View of the Mechanisms of UDP-N-Acetylglucosamine Enolpyruvyl Transferase (MurA) and 5-Enolpyruvylshikimate-3-phosphate Synthase (AroA) Derived from X-ray Structures of Their Tetrahedral Reaction Intermediate States.
J.Biol.Chem., 278:49215-49222, 2003

PubMed Abstract: UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) constitute the small enzyme family of enolpyruvyl transferases, which catalyze the chemically unusual reaction of enolpyruvyl transfer. MurA catalyzes the first step in the biosynthesis of the bacterial cell wall; AroA is the sixth enzyme of the shikimate pathway leading to the synthesis of aromatic compounds in numerous microorganisms and plants. Because both metabolic pathways are absent from mammals but essential for the growth of microorganisms, MurA and AroA are attractive targets for the development of novel antimicrobial drugs. We have determined the x-ray structures of the D305A mutant of Enterobacter cloacae MurA and the D313A mutant of Escherichia coli AroA, both of which crystallized in the presence of their substrates. The structures depict the tetrahedral reaction intermediate states of the enzymes and prove that, without the aspartate side chain, the overall addition-elimination reaction in both enzymes is halted after the addition step. The presented structures lead to a new view of the catalytic mechanism and, moreover, provide an ideal starting point for the rational design of potent inhibitors of MurA and AroA.

PubMed: 13129913
DOI: 10.1074/jbc.M309741200

実験手法

X-RAY DIFFRACTION (2.65 Å)