1Q3F

Uracil DNA glycosylase bound to a cationic 1-aza-2'-deoxyribose-containing DNA

1Q3F の概要

• エントリーDOI: 10.2210/pdb1q3f/pdb
• 関連するPDBエントリー: 1EMH 1SSP
• 分子名称: 5'-D(*TP*GP*TP*(NRI)P*AP*TP*CP*TP*T)-3', 5'-D(*AP*AP*AP*GP*AP*TP*AP*AP*CP*A)-3', Uracil-DNA glycosylase, ... (6 entities in total)
• 機能のキーワード: udg, dna repair, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Mitochondrion. Isoform 2: Nucleus: P13051
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 31407.98

構造登録者

Bianchet, M.A.,Seiple, L.A.,Jiang, Y.L.,Ichikawa, Y.,Amzel, L.M.,Stivers, J.T. (登録日: 2003-07-29, 公開日: 2004-03-23, 最終更新日: 2023-08-16)

主引用文献

Bianchet, M.A.,Seiple, L.A.,Jiang, Y.L.,Ichikawa, Y.,Amzel, L.M.,Stivers, J.T.
Electrostatic guidance of glycosyl cation migration along the reaction coordinate of uracil DNA glycosylase.
Biochemistry, 42:12455-12460, 2003

PubMed Abstract: The DNA repair enzyme uracil DNA glycosylase has been crystallized with a cationic 1-aza-2'-deoxyribose-containing DNA that mimics the ultimate transition state of the reaction in which the water nucleophile attacks the anomeric center of the oxacarbenium ion-uracil anion reaction intermediate. Comparison with substrate and product structures, and the previous structure of the intermediate determined by kinetic isotope effects, reveals an exquisite example of geometric strain, least atomic motion, and electrophile migration in biological catalysis. This structure provides a rare opportunity to reconstruct the detailed structural transformations that occur along an enzymatic reaction coordinate.

PubMed: 14580190
DOI: 10.1021/bi035372+

実験手法

X-RAY DIFFRACTION (1.9 Å)