1Q0H
Crystal structure of selenomethionine-labelled DXR in complex with fosmidomycin
Summary for 1Q0H
| Entry DOI | 10.2210/pdb1q0h/pdb |
| Related | 1Q0L 1Q0Q |
| Descriptor | 1-deoxy-D-xylulose 5-phosphate reductoisomerase, 3-[FORMYL(HYDROXY)AMINO]PROPYLPHOSPHONIC ACID, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (5 entities in total) |
| Functional Keywords | oxidoreductase |
| Biological source | Escherichia coli |
| Total number of polymer chains | 1 |
| Total formula weight | 46416.52 |
| Authors | Mac Sweeney, A.,Lange, R.,D'Arcy, A.,Douangamath, A.,Surivet, J.-P.,Oefner, C. (deposition date: 2003-07-16, release date: 2004-07-20, Last modification date: 2024-10-16) |
| Primary citation | Mac Sweeney, A.,Lange, R.,Fernandes, R.P.,Schulz, H.,Dale, G.E.,Douangamath, A.,Proteau, P.J.,Oefner, C. The crystal structure of E.coli 1-deoxy-D-xylulose-5-phosphate reductoisomerase in a ternary complex with the antimalarial compound fosmidomycin and NADPH reveals a tight-binding closed enzyme conformation. J.Mol.Biol., 345:115-127, 2005 Cited by PubMed Abstract: The key enzyme in the non-mevalonate pathway of isoprenoid biosynthesis, 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) has been shown to be the target enzyme of fosmidomycin, an antimalarial, antibacterial and herbicidal compound. Here we report the crystal structure of selenomethionine-labelled Escherichia coli DXR in a ternary complex with NADPH and fosmidomycin at 2.2 A resolution. The structure reveals a considerable conformational rearrangement upon fosmidomycin binding and provides insights into the slow, tight binding inhibition mode of the inhibitor. Although the inhibitor displays an unusual non-metal mediated mode of inhibition, which is an artefact most likely due to the low metal affinity of DXR at the pH used for crystallization, the structural data add valuable information for the rational design of novel DXR inhibitors. Using this structure together with the published structural data and the 1.9 A crystal structure of DXR in a ternary complex with NADPH and the substrate 1-deoxy-D-xylulose 5-phosphate, a model for the physiologically relevant tight-binding mode of inhibition is proposed. The structure of the substrate complex must be interpreted with caution due to the presence of a second diastereomer in the active site. PubMed: 15567415DOI: 10.1016/j.jmb.2004.10.030 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
Download full validation report
