1PZA

THE CRYSTAL STRUCTURES OF REDUCED PSEUDOAZURIN FROM ALCALIGENES FAECALIS S-6 AT TWO PH VALUES

Summary for 1PZA

Entry DOI	10.2210/pdb1pza/pdb
Related	1PAZ 1PZB
Descriptor	PSEUDOAZURIN, COPPER (II) ION (3 entities in total)
Functional Keywords	electron transfer(cuproprotein)
Biological source	Alcaligenes faecalis
Cellular location	Periplasm: P04377
Total number of polymer chains	1
Total formula weight	13447.06
Authors	Petratos, K. (deposition date: 1994-09-06, release date: 1994-11-30, Last modification date: 2024-02-14)
Primary citation	Vakoufari, E.,Wilson, K.S.,Petratos, K. The crystal structures of reduced pseudoazurin from Alcaligenes faecalis S-6 at two pH values. FEBS Lett., 347:203-206, 1994 Cited by PubMed Abstract: The structures of the reduced (Cu1+) blue-copper protein pseudoazurin from Alcaligenes faecalis strain S-6 are refined at pH 7.8 and 4.4 using X-ray diffraction data to 1.8 A resolution. The final R-factors for the high and low pH structures are 0.178 and 0.177, respectively. Comparing the reduced pseudoazurin at pH 7.8 with the oxidised (Cu2+) molecule, small changes are observed in the vicinity of the copper site and on the protein surface. At pH 4.4 the copper substituent imidazole of His81 rotates away from the metal with a concurrent movement of the latter towards the plane of the remaining three ligands (S gamma-Cys78, N delta 1-His40 and S delta-Met86) thus the geometry of the copper site becomes planar trigonal. PubMed: 8034003 DOI: 10.1016/0014-5793(94)00544-3 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.8 Å)

Structure validation