1PXW

Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii

Summary for 1PXW

Entry DOI	10.2210/pdb1pxw/pdb
Descriptor	LSU ribosomal protein L7AE (2 entities in total)
Functional Keywords	ribosome
Biological source	Pyrococcus abyssi
Cellular location	Cytoplasm : P62008
Total number of polymer chains	2
Total formula weight	28154.89
Authors	Charron, C.,Manival, X.,Charpentier, B.,Branlant, C.,Aubry, A. (deposition date: 2003-07-07, release date: 2004-01-27, Last modification date: 2024-02-14)
Primary citation	Charron, C.,Manival, X.,Charpentier, B.,Branlant, C.,Aubry, A. Purification, crystallization and preliminary X-ray diffraction data of L7Ae sRNP core protein from Pyrococcus abyssii. Acta Crystallogr.,Sect.D, 60:122-124, 2004 Cited by PubMed Abstract: The L7Ae sRNP core protein from Pyrococcus abyssii was crystallized using the sitting-drop vapour-diffusion method. Crystals were obtained in the presence of MgCl(2), PEG 2000 MME and acetate buffer at pH 4.0. A native data set has been collected at 2.9 A resolution using a rotating-anode generator at room temperature. Crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 70.7, b = 112.9, c = 34.8 A. There are two monomers of MW 14 200 Da per asymmetric unit and the packing density V(M) is 2.45 A(3) Da(-1). A molecular-replacement analysis gave solutions for the rotation and translation functions. PubMed: 14684904 DOI: 10.1107/S090744490302239X PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.94 Å)

Structure validation