1PSK
THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE
Summary for 1PSK
| Entry DOI | 10.2210/pdb1psk/pdb |
| Descriptor | ANTIBODY (3 entities in total) |
| Functional Keywords | fab, gd2-ganglioside, carbohydrate, melanoma, immunoglobulin |
| Biological source | Mus musculus (house mouse) More |
| Total number of polymer chains | 2 |
| Total formula weight | 45578.61 |
| Authors | Pichla, S.L.,Murali, R.,Burnett, R.M. (deposition date: 1997-03-03, release date: 1997-09-04, Last modification date: 2024-10-23) |
| Primary citation | Pichla, S.L.,Murali, R.,Burnett, R.M. The crystal structure of a Fab fragment to the melanoma-associated GD2 ganglioside. J.Struct.Biol., 119:6-16, 1997 Cited by PubMed Abstract: The GD2 ganglioside is a cell-surface component that appears on the surface of metastatic melanoma cells and is a marker for the progression of the disease. The ME36.1 monoclonal antibody binds to the GD2 ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a possible alternative therapy to radiation, which is often ineffective in late-stage melanoma. The crystal structure of the Fab fragment of ME36.1 has been determined using molecular replacement and refined to an R factor of 20.4% at 2.8 A resolution. The model has good geometry with root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7 degrees from ideal bond angles. The crystal structure of the ME36.1 Fab shows that its complementarity determining region forms a groove-shaped binding site rather than the pocket-type observed in other sugar binding Fabs. Molecular modeling has placed a four-residue sugar, representative of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by a network of hydrogen bonds that define the specificity of ME36.1 toward its antigen. PubMed: 9216084DOI: 10.1006/jsbi.1997.3857 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
Download full validation report
