1PSB

Solution structure of calcium loaded S100B complexed to a peptide from N-Terminal regulatory domain of NDR kinase.

Summary for 1PSB

• Entry DOI: 10.2210/pdb1psb/pdb
• Related: 1DT7 1MHO 1MQ1 1MWN 1QLK 1UWO
• Descriptor: S-100 protein, beta chain, Ndr Ser/Thr kinase-like protein (2 entities in total)
• Functional Keywords: helix-loop-helix, protein-peptide complex, metal binding protein
• Biological source: Bos taurus (cattle); More
• Cellular location: Cytoplasm: P02638; Nucleus: Q15208
• Total number of polymer chains: 4
• Total formula weight: 27567.26

Authors

Bhattacharya, S.,Large, E.,Heizmann, C.W.,Hemmings, B.,Chazin, W.J. (deposition date: 2003-06-21, release date: 2003-12-16, Last modification date: 2024-05-22)

Primary citation

Bhattacharya, S.,Large, E.,Heizmann, C.W.,Hemmings, B.,Chazin, W.J.
Structure of the Ca(2+)/S100B/NDR Kinase Peptide Complex: Insights into S100 Target Specificity and Activation of the Kinase.
Biochemistry, 42:14416-14426, 2003

PubMed Abstract: NDR, a nuclear serine/threonine kinase, belongs to the subfamily of Dbf2 kinases that is critical to the morphology and proliferation of cells. The activity of NDR kinase is modulated in a Ca(2+)/S100B-dependent manner by phosphorylation of Ser281 in the catalytic domain and Thr444 in the C-terminal regulatory domain. S100B, which is a member of the S100 subfamily of EF-hand proteins, binds to a basic/hydrophobic sequence at the junction of the N-terminal regulatory and catalytic domains (NDR(62-87)). Unlike calmodulin-dependent kinases, regulation of NDR by S100B is not associated with direct autoinhibition of the active site, but rather involves a conformational change in the catalytic domain triggered by Ca(2+)/S100B binding to the junction region. To gain further insight into the mechanism of activation of the kinase, studies have been carried out on Ca(2+)/S100B in complex with the intact N-terminal regulatory domain, NDR(1-87). Multidimensional heteronuclear NMR analysis showed that the binding mode and stoichiometry of a peptide fragment of NDR (NDR(62-87)) is the same as for the intact N-terminal regulatory domain. The solution structure of Ca(2+)/S100B and NDR(62-87) has been determined. One target molecule is found to associate with each subunit of the S100B dimer. The peptide adopts three turns of helix in the bound state, and the complex is stabilized by both hydrophobic and electrostatic interactions. These structural studies, in combination with available biochemical data, have been used to develop a model for calcium-induced activation of NDR kinase by S100B.

PubMed: 14661952
DOI: 10.1021/bi035089a

Experimental method

SOLUTION NMR