1POP

X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX

Summary for 1POP

• Entry DOI: 10.2210/pdb1pop/pdb
• Related PRD ID: PRD_000216
• Descriptor: PAPAIN, LEUPEPTIN, METHANOL, ... (4 entities in total)
• Functional Keywords: hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• Biological source: Carica papaya (papaya); More
• Total number of polymer chains: 2
• Total formula weight: 24647.93

Authors

Schroder, E.,Crawford, C. (deposition date: 1993-06-24, release date: 1993-10-31, Last modification date: 2024-10-23)

Primary citation

Schroder, E.,Phillips, C.,Garman, E.,Harlos, K.,Crawford, C.
X-ray crystallographic structure of a papain-leupeptin complex.
FEBS Lett., 315:38-42, 1993

PubMed Abstract: The three-dimensional structure of the papain-leupeptin complex has been determined by X-ray crystallography to a resolution of 2.1 A (overall R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts the S subsites of the papain active site and not the S' subsites; (ii) the 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25 sulphur atom of papain and is tetrahedrally coordinated; (iii) the 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes hydrogen bond contacts with Gln-19 and Cys-25.

PubMed: 8416808
DOI: 10.1016/0014-5793(93)81128-M

Experimental method

X-RAY DIFFRACTION (2.1 Å)