1PID

BOVINE DESPENTAPEPTIDE INSULIN

Summary for 1PID

• Entry DOI: 10.2210/pdb1pid/pdb
• Descriptor: DESPENTAPEPTIDE INSULIN (3 entities in total)
• Functional Keywords: hormone, glucose metabolism
• Biological source: Bos taurus (cattle); More
• Cellular location: Secreted: P01317 P01317
• Total number of polymer chains: 4
• Total formula weight: 10363.84

Authors

Holden, P.H.,Papiz, M.,Dodson, G.G. (deposition date: 1995-11-22, release date: 1996-12-07, Last modification date: 2024-10-23)

Primary citation

Brange, J.,Dodson, G.G.,Edwards, D.J.,Holden, P.H.,Whittingham, J.L.
A model of insulin fibrils derived from the x-ray crystal structure of a monomeric insulin (despentapeptide insulin).
Proteins, 27:507-516, 1997

PubMed Abstract: The crystal structure of despentapeptide insulin, a monomeric insulin, has been refined at 1.3 A spacing and subsequently used to predict and model the organization in the insulin fibril. The model makes use of the contacts in the densely packed despentapeptide insulin crystal, and takes into account other experimental evidence, including binding studies with Congo red. The dimensions of this model fibril correspond well with those measured experimentally, and the monomer-monomer contacts within the fibril are in accordance with the known physical chemistry of insulin fibrils. Using this model, it may be possible to predict mutations in insulin that might alleviate problems associated with fibril formation during insulin therapy.

PubMed: 9141131
DOI: 10.1002/(SICI)1097-0134(199704)27:4<507::AID-PROT4>3.3.CO;2-H

Experimental method

X-RAY DIFFRACTION (1.3 Å)