1P79
Crystal structure of a bulged RNA tetraplex: implications for a novel binding site in RNA tetraplex
Summary for 1P79
| Entry DOI | 10.2210/pdb1p79/pdb |
| Descriptor | 5'-R(*UP*GP*UP*GP*GP*U)-3', POTASSIUM ION (3 entities in total) |
| Functional Keywords | rna, tetraplex, bulge |
| Total number of polymer chains | 1 |
| Total formula weight | 2026.45 |
| Authors | Pan, B.,Xiong, Y.,Shi, K.,Sundaralingam, M. (deposition date: 2003-04-30, release date: 2003-11-04, Last modification date: 2024-02-14) |
| Primary citation | Pan, B.,Xiong, Y.,Shi, K.,Sundaralingam, M. Crystal Structure of a Bulged RNA Tetraplex at 1.1 A Resolution: Implications for a Novel Binding Site in RNA Tetraplex STRUCTURE, 11:1423-1430, 2003 Cited by PubMed Abstract: Bulges are an important structural motif in RNA and can be used as recognition and interaction sites in RNA-protein interaction and RNA-RNA interaction. Here we report the first crystal structure of a bulged RNA tetraplex at 1.1 A resolution. The hexamer r(U)(BrdG)r(UGGU) forms a parallel tetraplex with the uridine sandwiched by guanines bulging out. The bulged uridine adopts the syn glycosidic conformation and its O2 and N3 atoms face outwards, serving as an effective recognition and interaction site. The bulge formation both widens the groove width and changes the groove hydrogen-bonding pattern on its 5' side. However, the bulge does not make any bends or kinks in the tetraplex structure. The present study demonstrates the dramatic difference between uridine and guanine in forming tetraplex structure. In addition, both G(syn) tetrad and G(anti) tetrad have been observed. They display the same base-pairing pattern and similar C1'-C1' distance but different hydrogen-bonding patterns in the groove. PubMed: 14604532DOI: 10.1016/j.str.2003.09.017 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.1 Å) |
Structure validation
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