1P79
Crystal structure of a bulged RNA tetraplex: implications for a novel binding site in RNA tetraplex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.8985 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 35.014, 35.014, 22.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.100 |
R-factor | 0.12733 |
Rwork | 0.127 |
R-free | 0.13600 * |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.550 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.037 * | 0.088 * |
Total number of observations | 206863 * | |
Number of reflections | 10280 * | |
<I/σ(I)> | 23 | |
Completeness [%] | 95.3 | 71.9 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | spermine, potassium chloride, MPD, cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | spermine | ||
2 | 1 | 1 | potassium chloride | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 1 | cacodylate | ||
5 | 1 | 2 | potassium chloride | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.0 |
2 | 1 | drop | spermine tetrahydrochloride | 12 (mM) | |
3 | 1 | drop | 80 (mM) | ||
4 | 1 | drop | MPD | 10 (%(v/v)) | |
5 | 1 | drop | 50 (mM) | ||
6 | 1 | reservoir | 10 (%) |