1OTV

PqqC, Pyrroloquinolinquinone Synthase C

Summary for 1OTV

• Entry DOI: 10.2210/pdb1otv/pdb
• Descriptor: Coenzyme PQQ synthesis protein C (2 entities in total)
• Functional Keywords: seven helix bundle, dimer, pqq biosynthesis enzyme, biosynthetic protein
• Biological source: Klebsiella pneumoniae
• Total number of polymer chains: 2
• Total formula weight: 60284.23

Authors

Magnusson, O.T.,Toyama, H.,Saeki, M.,Rojas, A.,Reed, J.C.,Adachi, O.,Klinman, J.P.,SChwarzenbacher, R. (deposition date: 2003-03-23, release date: 2004-05-11, Last modification date: 2024-02-14)

Primary citation

Magnusson, O.T.,Toyama, H.,Saeki, M.,Rojas, A.,Reed, J.C.,Adachi, O.,Liddington, R.C.,Klinman, J.P.,Schwarzenbacher, R.
Quinone Biogenesis: Structure and Mechanism of PqqC, the Final Catalyst in the Production of Pyrroloquinoline Quinone.
Proc.Natl.Acad.Sci.USA, 101:7913-7918, 2004

PubMed Abstract: The biosynthesis of pyrroloquinoline quinone (PQQ), a vitamin and redox cofactor of quinoprotein dehydrogenases, is facilitated by an unknown pathway that requires the expression of six genes, pqqA to -F. PqqC, the protein encoded by pqqC, catalyzes the final step in the pathway in a reaction that involves ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ. Herein, we describe the crystal structures of PqqC and its complex with PQQ and determine the stoichiometry of H2O2 formation and O2 uptake during the reaction. The PqqC structure(s) reveals a compact seven-helix bundle that provides the scaffold for a positively charged active site cavity. Product binding induces a large conformational change, which results in the active site recruitment of amino acid side chains proposed to play key roles in the catalytic mechanism. PqqC is unusual in that it transfers redox equivalents to molecular oxygen without the assistance of a redox active metal or cofactor. The structure of the enzyme-product complex shows additional electron density next to R179 and C5 of PQQ, which can be modeled as O2 or H2O2, indicating a site for oxygen binding. We propose a reaction sequence that involves base-catalyzed cyclization and a series of quinone-quinol tautomerizations that are followed by cycles of O2/H2O2-mediated oxidations.

PubMed: 15148379
DOI: 10.1073/pnas.0402640101

Experimental method

X-RAY DIFFRACTION (2.1 Å)