1OSH

A Chemical, Genetic, and Structural Analysis of the nuclear bile acid receptor FXR

1OSH の概要

• エントリーDOI: 10.2210/pdb1osh/pdb
• 分子名称: Bile acid receptor, METHYL 3-{3-[(CYCLOHEXYLCARBONYL){[4'-(DIMETHYLAMINO)BIPHENYL-4-YL]METHYL}AMINO]PHENYL}ACRYLATE (3 entities in total)
• 機能のキーワード: nuclear receptor, ligand binding domain, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Probable): Q96RI1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27583.72

構造登録者

Downes, M.,Verdecia, M.A.,Roecker, A.J.,Hughes, R.,Hogenesch, J.B.,Kast-Woelbern, H.R.,Bowman, M.E.,Ferrer, J.-L.,Anisfeld, A.M.,Edwards, P.A.,Rosenfeld, J.M.,Alvarez, J.G.A.,Noel, J.P.,Nicolaou, K.C.,Evans, R.M. (登録日: 2003-03-19, 公開日: 2003-09-23, 最終更新日: 2024-02-14)

主引用文献

Downes, M.,Verdecia, M.A.,Roecker, A.J.,Hughes, R.,Hogenesch, J.B.,Kast-Woelbern, H.R.,Bowman, M.E.,Ferrer, J.-L.,Anisfeld, A.M.,Edwards, P.A.,Rosenfeld, J.M.,Alvarez, J.G.,Noel, J.P.,Nicolaou, K.C.,Evans, R.M.
A chemical, genetic, and structural analysis of the nuclear bile acid receptor FXR
Mol.Cell, 11:1079-1092, 2003

PubMed Abstract: The farnesoid X receptor (FXR) functions as a bile acid (BA) sensor coordinating cholesterol metabolism, lipid homeostasis, and absorption of dietary fats and vitamins. However, BAs are poor reagents for characterizing FXR functions due to multiple receptor independent properties. Accordingly, using combinatorial chemistry we evolved a small molecule agonist termed fexaramine with 100-fold increased affinity relative to natural compounds. Gene-profiling experiments conducted in hepatocytes with FXR-specific fexaramine versus the primary BA chenodeoxycholic acid (CDCA) produced remarkably distinct genomic targets. Highly diffracting cocrystals (1.78 A) of fexaramine bound to the ligand binding domain of FXR revealed the agonist sequestered in a 726 A(3) hydrophobic cavity and suggest a mechanistic basis for the initial step in the BA signaling pathway. The discovery of fexaramine will allow us to unravel the FXR genetic network from the BA network and selectively manipulate components of the cholesterol pathway that may be useful in treating cholesterol-related human diseases.

PubMed: 12718892
DOI: 10.1016/S1097-2765(03)00104-7

実験手法

X-RAY DIFFRACTION (1.8 Å)