1OS5

Crystal structure of HCV NS5B RNA polymerase complexed with a novel non-competitive inhibitor.

1OS5 の概要

• エントリーDOI: 10.2210/pdb1os5/pdb
• 分子名称: Hepatitis C virus NS5B RNA polymerase, 3-(4-AMINO-2-TERT-BUTYL-5-METHYL-PHENYLSULFANYL)-6-CYCLOPENTYL-4-HYDROXY-6-[2-(4-HYDROXY-PHENYL)-ETHYL]-5,6-DIHYDRO-PYRAN-2-ONE (3 entities in total)
• 機能のキーワード: enzyme-inhibitor complex, transferase
• 由来する生物種: Hepatitis C virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 64658.13

構造登録者

Love, R.A.,Parge, H.E.,Yu, X.,Hickey, M.J.,Diehl, W.,Gao, J.,Wriggers, H.,Ekker, A.,Wang, L.,Thomson, J.A.,Dragovich, P.S.,Fuhrman, S.A. (登録日: 2003-03-18, 公開日: 2004-03-18, 最終更新日: 2024-04-03)

主引用文献

Love, R.A.,Parge, H.E.,Yu, X.,Hickey, M.J.,Diehl, W.,Gao, J.,Wriggers, H.,Ekker, A.,Wang, L.,Thomson, J.A.,Dragovich, P.S.,Fuhrman, S.A.
Crystallographic identification of a noncompetitive inhibitor binding site on the hepatitis C virus NS5B RNA polymerase enzyme.
J.Virol., 77:7575-7581, 2003

PubMed Abstract: The virus-encoded nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is an RNA-dependent RNA polymerase and is absolutely required for replication of the virus. NS5B exhibits significant differences from cellular polymerases and therefore has become an attractive target for anti-HCV therapy. Using a high-throughput screen, we discovered a novel NS5B inhibitor that binds to the enzyme noncompetitively with respect to nucleotide substrates. Here we report the crystal structure of NS5B complexed with this small molecule inhibitor. Unexpectedly, the inhibitor is bound within a narrow cleft on the protein's surface in the "thumb" domain, about 30 A from the enzyme's catalytic center. The interaction between this inhibitor and NS5B occurs without dramatic changes to the structure of the protein, and sequence analysis suggests that the binding site is conserved across known HCV genotypes. Possible mechanisms of inhibition include perturbation of protein dynamics, interference with RNA binding, and disruption of enzyme oligomerization.

PubMed: 12805457
DOI: 10.1128/JVI.77.13.7575-7581.2003

実験手法

X-RAY DIFFRACTION (2.2 Å)