Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1OOF

Complex of Drosophila odorant binding protein LUSH with ethanol

Summary for 1OOF
Entry DOI10.2210/pdb1oof/pdb
Related1OOG 1OOH 1OOI
Descriptorodorant binding protein LUSH, ACETATE ION, ETHANOL, ... (4 entities in total)
Functional Keywordslush, alcohol, odorant binding, transport protein
Biological sourceDrosophila melanogaster (fruit fly)
Cellular locationSecreted: O02372
Total number of polymer chains2
Total formula weight28759.33
Authors
Kruse, S.W.,Zhao, R.,Smith, D.P.,Jones, D.N.M. (deposition date: 2003-03-03, release date: 2003-09-02, Last modification date: 2011-07-13)
Primary citationKruse, S.W.,Zhao, R.,Smith, D.P.,Jones, D.N.M.
Structure of a specific alcohol-binding site defined by the odorant binding protein LUSH from Drosophila melanogaster
Nat.Struct.Biol., 10:694-700, 2003
Cited by
PubMed Abstract: We have solved the high-resolution crystal structures of the Drosophila melanogaster alcohol-binding protein LUSH in complex with a series of short-chain n-alcohols. LUSH is the first known nonenzyme protein with a defined in vivo alcohol-binding function. The structure of LUSH reveals a set of molecular interactions that define a specific alcohol-binding site. A group of amino acids, Thr57, Ser52 and Thr48, form a network of concerted hydrogen bonds between the protein and the alcohol that provides a structural motif to increase alcohol-binding affinity at this site. This motif seems to be conserved in a number of mammalian ligand-gated ion channels that are directly implicated in the pharmacological effects of alcohol. Further, these sequences are found in regions of ion channels that are known to confer alcohol sensitivity. We suggest that the alcohol-binding site in LUSH represents a general model for alcohol-binding sites in proteins.
PubMed: 12881720
DOI: 10.1038/nsb960
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.49 Å)
Structure validation

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon