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1OOF

Complex of Drosophila odorant binding protein LUSH with ethanol

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-BM
Synchrotron siteAPS
Beamline19-BM
Temperature [K]105
Detector technologyCCD
Collection date2002-02-21
DetectorSBC-1
Wavelength(s)0.97895
Spacegroup nameP 43
Unit cell lengths46.942, 46.942, 111.540
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.000 - 1.490
R-factor0.18135
Rwork0.178
R-free0.21000

*

Structure solution methodMAD
RMSD bond length0.015
RMSD bond angle1.445
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareREFMAC (5.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0001.540
High resolution limit [Å]1.4901.490
Rmerge0.0390.196
Total number of observations119487

*

Number of reflections38748
<I/σ(I)>25.42.5
Completeness [%]98.299.5
Redundancy3.11.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

4

*

4 or 18

*

PEG 4000, sodium acetate, ethanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirsodium acetate100 (mM)pH4.0
21reservoirPEG400025-30 (%(w/v))
31reservoiralcohol0.3 (%(v/v))

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