1OJL
Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding
Summary for 1OJL
| Entry DOI | 10.2210/pdb1ojl/pdb |
| Descriptor | TRANSCRIPTIONAL REGULATORY PROTEIN ZRAR, PHOSPHATE ION, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total) |
| Functional Keywords | response regulator, two component system, aaa domain, ntrc family, dna-binding, transcription regulation |
| Biological source | SALMONELLA TYPHIMURIUM |
| Total number of polymer chains | 6 |
| Total formula weight | 203413.62 |
| Authors | Sallai, L.,Tucker, P.A. (deposition date: 2003-07-10, release date: 2005-05-19, Last modification date: 2024-05-08) |
| Primary citation | Sallai, L.,Tucker, P.A. Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR. J. Struct. Biol., 151:160-170, 2005 Cited by PubMed Abstract: The sigma(54)-dependent transcription in bacteria is associated with various stress and growth conditions. Activators of the sigma(54) protein contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both prokaryotic and eukaryotic cells. We describe the X-ray structure of an N-terminal domain deletion of the ZraR protein from Salmonella typhimurium, which is a homologue of the general nitrogen regulatory protein NtrC, at 3A resolution. The structure reveals a hexameric ring that is typical for AAA+ containing proteins but which differs from the heptameric ring found in the crystal structure of the AAA+ domain of NtrC1 from Aquifex aeolicus. The dimerisation interface between DNA-binding domains observed in the crystal structure suggests that dodecamers, rather than hexamers, might be the functionally important oligomer. PubMed: 16005641DOI: 10.1016/j.jsb.2005.05.006 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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