1OJL
Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 107.440, 114.740, 187.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.25457 |
| Rwork | 0.252 |
| R-free | 0.30833 |
| Structure solution method | SIRAS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 46186 | |
| <I/σ(I)> | 10.9 | 2.6 |
| Completeness [%] | 97.5 | 87.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | SITTING DROP VAPOUR DIFFUSION, WELL:100MM HEPES PH8.2 9% ISOPROPANOL, 3% METHANOL, 200MM NACL PROTEIN: 10-12MG/ML ,20MM CHES PH9.5,2MM DTT ,0.1MM EDTA. DROP 1:0.6 PROTEIN:WELL, pH 8.00 |
