1OKE

Crystal structure of the dengue 2 virus envelope protein in complex with n-octyl-beta-D-glucoside

Replaces:  1OAM

Summary for 1OKE

• Entry DOI: 10.2210/pdb1oke/pdb
• Related: 1L9K 1OAM 1OAN 1OK8
• Descriptor: MAJOR ENVELOPE PROTEIN E, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: viral protein, membrane fusion, flavivirus, fusion peptide, low-ph conformational change, class 2 fusion protein
• Biological source: DENGUE VIRUS TYPE 2
• Cellular location: Capsid protein C: Virion (Potential). Peptide pr: Secreted (By similarity). Small envelope protein M: Virion membrane; Multi-pass membrane protein (By similarity). Envelope protein E: Virion membrane; Multi- pass membrane protein (By similarity). Non-structural protein 1: Secreted. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P12823
• Total number of polymer chains: 2
• Total formula weight: 90131.30

Authors

Modis, Y.,Harrison, S.C. (deposition date: 2003-07-22, release date: 2003-07-24, Last modification date: 2024-11-06)

Primary citation

Modis, Y.,Ogata, S.,Clements, D.,Harrison, S.C.
A Ligand-Binding Pocket in the Dengue Virus Envelope Glycoprotein.
Proc.Natl.Acad.Sci.USA, 100:6986-, 2003

PubMed Abstract: Dengue virus is an emerging global health threat. Its major envelope glycoprotein, E, mediates viral attachment and entry by membrane fusion. A crystal structure of the soluble ectodomain of E from dengue virus type 2 reveals a hydrophobic pocket lined by residues that influence the pH threshold for fusion. The pocket, which accepts a hydrophobic ligand, opens and closes through a conformational shift in a beta-hairpin at the interface between two domains. These features point to a structural pathway for the fusion-activating transition and suggest a strategy for finding small-molecule inhibitors of dengue and other flaviviruses.

PubMed: 12759475
DOI: 10.1073/PNAS.0832193100

Experimental method

X-RAY DIFFRACTION (2.4 Å)