1OKE

Crystal structure of the dengue 2 virus envelope protein in complex with n-octyl-beta-D-glucoside

Replaces:  1OAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-15
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	81.602, 81.602, 287.408
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 2.400
R-factor	0.263
Rwork	0.263
R-free	0.29400
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.069	0.289
Number of reflections	42998
<I/σ(I)>	26.2	3.3
Completeness [%]	96.8	74
Redundancy	5.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		11% PEG 8K, 1M NACL, 0.1M TRIS/HCL PH 9.0, 20% GLYCEROL,0.5% OCTYLGLUCOSIDE
1		8.5		11% PEG 8K, 1M NACL, 0.1M TRIS/HCL PH 9.0, 20% GLYCEROL,0.5% OCTYLGLUCOSIDE