1O80
Crystal structure of IP-10 H-Form
Summary for 1O80
Entry DOI | 10.2210/pdb1o80/pdb |
Related | 1LV9 1O7Y 1O7Z |
Descriptor | SMALL INDUCIBLE CYTOKINE B10 (2 entities in total) |
Functional Keywords | chemokine, interferon induction, chemotaxis, inflammatory response |
Biological source | HOMO SAPIENS (HUMAN) |
Cellular location | Secreted: P02778 |
Total number of polymer chains | 2 |
Total formula weight | 17274.69 |
Authors | Swaminathan, G.J.,Holloway, D.E.,Papageorgiou, A.C.,Acharya, K.R. (deposition date: 2002-11-20, release date: 2003-05-08, Last modification date: 2024-10-23) |
Primary citation | Swaminathan, G.J.,Holloway, D.E.,Colvin, R.A.,Campanella, G.K.,Papageorgiou, A.C.,Luster, A.D.,Acharya, K.R. Crystal Structures of Oligomeric Forms of the Ip-10/Cxcl10 Chemokine Structure, 11:521-, 2003 Cited by PubMed Abstract: We have determined the structure of wild-type IP-10 from three crystal forms. The crystals provide eight separate models of the IP-10 chain, all differing substantially from a monomeric IP-10 variant examined previously by NMR spectroscopy. In each crystal form, IP-10 chains form conventional beta sheet dimers, which, in turn, form a distinct tetrameric assembly. The M form tetramer is reminiscent of platelet factor 4, whereas the T and H forms feature a novel twelve-stranded beta sheet. Analytical ultracentrifugation indicates that, in free solution, IP-10 exists in a monomer-dimer equilibrium with a dissociation constant of 9 microM. We propose that the tetrameric structures may represent species promoted by the binding of glycosaminoglycans. The binding sites for several IP-10-neutralizing mAbs have also been mapped. PubMed: 12737818DOI: 10.1016/S0969-2126(03)00070-4 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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