1O80
Crystal structure of IP-10 H-Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-25 |
Detector | ADSC CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 84.560, 84.560, 116.967 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 * - 2.000 |
R-factor | 0.264 |
Rwork | 0.264 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rhp |
RMSD bond length | 0.009 |
RMSD bond angle | 24.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.078 | 0.520 |
Total number of observations | 219378 * | |
Number of reflections | 17139 | |
<I/σ(I)> | 20.1 | 3.3 |
Completeness [%] | 98.4 | 99.8 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.75 | 16 * | 10MG/ML PROTEIN, 0.1M TRIS-HCL BUFFER, PH 8.75, 3.3M SODIUM FORMATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.75 |
3 | 1 | reservoir | sodium formate | 3.3 (M) |