1NZE

Crystal structure of PsbQ polypeptide of photosystem II from higher plants

Summary for 1NZE

• Entry DOI: 10.2210/pdb1nze/pdb
• Related: 1FE1 1ILX
• Descriptor: Oxygen-evolving enhancer protein 3, ZINC ION (3 entities in total)
• Functional Keywords: photosystem ii, oxygen-enhancer evolving complex, water oxidizing complex, psbq, oee3, oxidoreductase
• Biological source: Spinacia oleracea (spinach)
• Cellular location: Plastid, chloroplast thylakoid membrane: P12301
• Total number of polymer chains: 1
• Total formula weight: 16610.15

Authors

Calderone, V.,Trabucco, M.,Vujicic, A.,Battistutta, R.,Giacometti, G.M.,Andreucci, F.,Barbato, R.,Zanotti, G. (deposition date: 2003-02-17, release date: 2003-08-26, Last modification date: 2024-02-14)

Primary citation

Calderone, V.,Trabucco, M.,Vujicic, A.,Battistutta, R.,Giacometti, G.M.,Andreucci, F.,Barbato, R.,Zanotti, G.
Crystal structure of the PsbQ protein of photosystem II from higher plants
Embo Rep., 4:900-905, 2003

PubMed Abstract: The smallest extrinsic polypeptide of the water-oxidizing complex (PsbQ) was extracted and purified from spinach (Spinacia oleracea) photosystem II (PSII) membranes. It was then crystallized in the presence of Zn(2+) and its structure was determined by X-ray diffraction at 1.95-A resolution using the multi-wavelength anomalous diffraction method, with the zinc as the anomalous scatterer. The crystal structure shows that the core of the protein is a four-helix bundle, whereas the amino-terminal portion, which possibly interacts with the photosystem core, is not visible in the crystal. The distribution of positive and negative charges on the protein surface might explain the ability of PsbQ to increase the binding of Cl(-) and Ca(2+) and make them available to PSII.

PubMed: 12949587
DOI: 10.1038/sj.embor.embor923

Experimental method

X-RAY DIFFRACTION (1.95 Å)