1NZE

Crystal structure of PsbQ polypeptide of photosystem II from higher plants

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.93, 1.282, 1.284
Spacegroup name	P 32 2 1
Unit cell lengths	50.282, 50.282, 94.067
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.550 - 1.950
R-factor	0.227
Rwork	0.227
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.010 *
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000 *	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.110	0.330
Number of reflections	10333	1340 *
<I/σ(I)>	3.3	1.2
Completeness [%]	98.1	90.9
Redundancy	8.9 *	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6 *	20 *	50 mM Tris, 50 mM NaCl, 5 mM Zn2+, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	Vapor diffusion *	4.6 *	20 *	50 mM Tris, 50 mM NaCl, 5 mM Zn2+, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	Vapor diffusion *	4.6 *	20 *	50 mM Tris, 50 mM NaCl, 5 mM Zn2+, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-24 (mg/ml)
2	1	reservoir	PEG3350	25-30 (%(w/v))	or PDB4000
3	1	reservoir		5 (mM)	pH4.6