1NLY

Crystal structure of the traffic ATPase of the Helicobacter pylori type IV secretion system in complex with ATPgammaS

Summary for 1NLY

• Entry DOI: 10.2210/pdb1nly/pdb
• Related: 1G6O
• Descriptor: virB11 homolog, MAGNESIUM ION, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (6 entities in total)
• Functional Keywords: virb11 atpase, bacterial type iv secretion, atpgammas, hp0525, helicobacter pylori, hydrolase
• Biological source: Helicobacter pylori
• Total number of polymer chains: 2
• Total formula weight: 77409.83

Authors

Savvides, S.N.,Yeo, H.J.,Beck, M.R.,Blaesing, F.,Lurz, R.,Lanka, E.,Buhrdorf, R.,Fischer, W.,Haas, R.,Waksman, G. (deposition date: 2003-01-08, release date: 2003-05-06, Last modification date: 2024-10-16)

Primary citation

Savvides, S.N.,Yeo, H.J.,Beck, M.R.,Blaesing, F.,Lurz, R.,Lanka, E.,Buhrdorf, R.,Fischer, W.,Haas, R.,Waksman, G.
VirB11 ATPases are dynamic hexameric assemblies: New insights into bacterial type IV secretion
Embo J., 22:1969-1980, 2003

PubMed Abstract: The coupling of ATP binding/hydrolysis to macromolecular secretion systems is crucial to the pathogenicity of Gram-negative bacteria. We reported previously the structure of the ADP-bound form of the hexameric traffic VirB11 ATPase of the Helicobacter pylori type IV secretion system (named HP0525), and proposed that it functions as a gating molecule at the inner membrane, cycling through closed and open forms regulated by ATP binding/hydrolysis. Here, we combine crystal structures with analytical ultracentrifugation experiments to show that VirB11 ATPases indeed function as dynamic hexameric assemblies. In the absence of nucleotide, the N-terminal domains exhibit a collection of rigid-body conformations. Nucleotide binding 'locks' the hexamer into a symmetric and compact structure. We propose that VirB11s use the mechanical leverage generated by such nucleotide-dependent conformational changes to facilitate the export of substrates or the assembly of the type IV secretion apparatus. Biochemical characterization of mutant forms of HP0525 coupled with electron microscopy and in vivo assays support such hypothesis, and establish the relevance of VirB11s ATPases as drug targets against pathogenic bacteria.

PubMed: 12727865
DOI: 10.1093/emboj/cdg223

Experimental method

X-RAY DIFFRACTION (2.8 Å)