1NCO

STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN

Summary for 1NCO

• Entry DOI: 10.2210/pdb1nco/pdb
• Descriptor: HOLO-NEOCARZINOSTATIN, (4R)-2-METHYLPENTANE-2,4-DIOL, NEOCARZINOSTATIN-CHROMOPHORE, ... (4 entities in total)
• Functional Keywords: antibacterial and antitumor protein
• Biological source: Streptomyces carzinostaticus
• Total number of polymer chains: 2
• Total formula weight: 22977.92

Authors

Kim, K.-H.,Kwon, B.-M.,Myers, A.G.,Rees, D.C. (deposition date: 1993-07-13, release date: 1993-10-31, Last modification date: 2024-10-09)

Primary citation

Kim, K.H.,Kwon, B.M.,Myers, A.G.,Rees, D.C.
Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex.
Science, 262:1042-1046, 1993

PubMed Abstract: Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. The chromophore pi-face interacts with the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition during chromophore activation, are unexposed. The position of the amino group of the chromophore carbohydrate relative to C-12 supports the idea that the amino group plays a role in thiol activation.

PubMed: 8235619

Experimental method

X-RAY DIFFRACTION (1.8 Å)