1N7S

High Resolution Structure of a Truncated Neuronal SNARE Complex

1N7S の概要

• エントリーDOI: 10.2210/pdb1n7s/pdb
• 分子名称: vesicle-associated membrane protein 2, Syntaxin 1A, SNAP-25A, ... (7 entities in total)
• 機能のキーワード: neuronal snare protein complex, four helix bundle, transport protein
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P63045 P32851; Cytoplasm, perinuclear region : P60881 P60881
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 32097.08

構造登録者

Ernst, J.A.,Brunger, A.T. (登録日: 2002-11-16, 公開日: 2002-12-27, 最終更新日: 2024-02-14)

主引用文献

Ernst, J.A.,Brunger, A.T.
High Resolution Structure, Stability, and Synaptotagmin Binding of a Truncated Neuronal SNARE Complex
J.Biol.Chem., 278:8630-8636, 2003

PubMed Abstract: The structure of a truncated SNARE complex has been solved to 1.4-A resolution revealing a stabilizing salt bridge, sites of hydration, and conformational variability of the ionic central layer that were not observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid binding and denatures at a lower temperature than longer complexes as assessed by SDS and circular dichroism thermal melts. The truncated SNARE complex is monomeric, and it retains binding to synaptotagmin I.

PubMed: 12496247
DOI: 10.1074/jbc.M211889200

実験手法

X-RAY DIFFRACTION (1.45 Å)