1N7D

Extracellular domain of the LDL receptor

Summary for 1N7D

• Entry DOI: 10.2210/pdb1n7d/pdb
• Descriptor: Low-density lipoprotein receptor, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: ldl-receptor, familial hypercholesterolemia, ldl, cholesterol metabolism, lipid transport
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 88314.84

Authors

Rudenko, G.,Henry, L.,Henderson, K.,Ichtchenko, K.,Brown, M.S.,Goldstein, J.L.,Deisenhofer, J. (deposition date: 2002-11-13, release date: 2003-01-21, Last modification date: 2025-11-12)

Primary citation

Rudenko, G.,Henry, L.,Henderson, K.,Ichtchenko, K.,Brown, M.S.,Goldstein, J.L.,Deisenhofer, J.
Structure of the LDL receptor extracellular domain at endosomal pH
Science, 298:2353-2358, 2002

PubMed Abstract: The low-density lipoprotein receptor mediates cholesterol homeostasis through endocytosis of lipoproteins. It discharges its ligand in the endosome at pH < 6. In the crystal structure at pH = 5.3, the ligand-binding domain (modules R2 to R7) folds back as an arc over the epidermal growth factor precursor homology domain (the modules A, B, beta propeller, and C). The modules R4 and R5, which are critical for lipoprotein binding, associate with the beta propeller via their calcium-binding loop. We propose a mechanism for lipoprotein release in the endosome whereby the beta propeller functions as an alternate substrate for the ligand-binding domain, binding in a calcium-dependent way and promoting lipoprotein release.

PubMed: 12459547
DOI: 10.1126/science.1078124

Experimental method

X-RAY DIFFRACTION (3.7 Å)